Publications

A reference tissue atlas for the human kidney.

Hansen J, Sealfon R, Menon R, Eadon MT, Lake BB, Steck B, Anjani K, Parikh S, Sigdel TK, Zhang G, Velickovic D, Barwinska D, Alexandrov T, Dobi D, Rashmi P, Otto EA, Rivera M, Rose MP, Anderton CR, Shapiro JP, Pamreddy A, Winfree S, Xiong Y, He Y, de Boer IH, Hodgin JB, Barisoni L, Naik AS, Sharma K, Sarwal MM, Zhang K, Himmelfarb J, Rovin B, El-Achkar TM, Laszik Z, He JC, Dagher PC, Valerius MT, Jain S, Satlin LM, Troyanskaya OG, Kretzler M, Iyengar R, Azeloglu EU; Kidney Precision Medicine Project.
Sci Adv. 2022 Jun 10;8(23):eabn4965. doi: 10.1126/sciadv.abn4965. Epub 2022 Jun 8.
PMID: 35675394

Proteomic cellular signatures of kinase inhibitor-induced cardiotoxicity

Xiong Y , Liu T, Chen T, Hansen J, Hu B, Chen Y, Jayaraman G, Schürer S, Vidovic D, Goldfarb J, Sobie EA, Birtwistle MR , Iyengar R, Li H, Azeloglu EU.
Sci Data. 2022 Jan 20;9(1):18.

A library of induced pluripotent stem cells from clinically well-characterized, diverse healthy human individuals

Schaniel C, Dhanan P, Hu B, Xiong Y, Raghunandan T, Gonzalez DM, Dariolli R, D'Souza SL , Yadaw AS, Hansen J, Jayaraman G, Mathew B, Machado M, Berger SI , Tripodi J, Najfeld V, Garg J, Miller M,Surlyn CS, Michelis KC, Tangirala NC, Weerahandi H, Thomas DC, Kristin G Beaumont KG, Sebra R, Mahajan M , Schadt E, Vidovic D, Schürer SC, Goldfarb J , Azeloglu EU, Birtwistle MR, Sobie EA , Kovacic JC, Dubois NC, Iyengar R.
Stem Cell Reports. 2021 Dec 14;16(12):3036-3049.

Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion

Alves CJ, Dariolli R, Haydak J, Kang S, Hannah T, Wiener RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Dias RA, Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de Mendonça JP, Azeloglu EU, Zou H, Friedel RH.
Nat Commun. 2021 Oct 14;12(1):6019.

Modeling the Glomerular Filtration Barrier and Intercellular Crosstalk

Ebefors K, Lassén E, Anandakrishnan N, Azeloglu EU, Daehn IS.
Front Physiol. 2021 Jun 2;12:689083.

DACH1 protects podocytes from experimental diabetic injury and modulates PTIP-H3K4Me3 activity.

Cao A, Li J, Asadi M, Basgen JM, Zhu B, Yi Z, Jiang S, Doke T, El Shamy O, Patel N, Cravedi P, Azeloglu EU, Campbell KN, Menon M, Coca S, Zhang W, Wang H, Zen K, Liu Z, Murphy B, He JC, D'Agati VD, Susztak K, Kaufman L.
J Clin Invest. 2021 May 17;131(10):141279.

Protein structure-based gene expression signatures.

Rahman R, Zatorski N, Hansen J, Xiong Y, van Hasselt JGC, Sobie EA, Birtwistle MR, Azeloglu EU, Iyengar R, Schlessinger A.
Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2014866118.

Deficiency in nebulin repeats of sarcomeric nebulette is detrimental for cardiomyocyte tolerance to exercise and biomechanical stress

Vejandla RM, Orgil BO, Alberson NR, Li N, Munkhsaikhan U, Khuchua Z, Martherus R, Azeloglu EU, Xu F, Lu L, Towbin JA, Purevjav E.
Am J Physiol Heart Circ Physiol. 2021 May 1;320(5):H2130-H2146.

Rationale and design of the Kidney Precision Medicine Project.

de Boer IH, Alpers CE, Azeloglu EU, Balis UGJ, Barasch JM, Barisoni L, Blank KN, Bomback AS, Brown K, Dagher PC, Dighe AL, Eadon MT, El-Achkar TM, Gaut JP, Hacohen N, He Y, Hodgin JB, Jain S, Kellum JA, Kiryluk K, Knight R, Laszik ZG, Lienczewski C, Mariani LH, McClelland RL, Menez S, Moledina DG, Mooney SD, O'Toole JF, Palevsky PM, Parikh CR, Poggio ED, Rosas SE, Rosengart MR, Sarwal MM, Schaub JA, Sedor JR, Sharma K, Steck B, Toto RD, Troyanskaya OG, Tuttle KR, Vazquez MA, Waikar SS, Williams K, Wilson FP, Zhang K, Iyengar R, Kretzler M, Himmelfarb J; Kidney Precision Medicine Project.
Kidney Int. 2021 Mar;99(3):498-510.

Nicotine, smoking, podocytes, and diabetic nephropathy.

Jaimes EA, Zhou MS, Siddiqui M, Rezonzew G, Tian R, Seshan SV, Muwonge AN, Wong NJ, Azeloglu EU, Fornoni A, Merscher S, Raij L.
Am J Physiol Renal Physiol. 2021 Mar 1;320(3):F442-F453.

A Multimodal and Integrated Approach to Interrogate Human Kidney Biopsies with Rigor and Reproducibility: Guidelines from the Kidney Precision Medicine Project.

El-Achkar TM, Eadon MT, Menon R, Lake BB, Sigdel TK, Alexandrov T, Parikh S, Zhang G, Dobib D, Dunn KW, Otto EA, Anderton CR, Carson JM, Luo J, Park C, Hamidi H, Zhou J, Hoover P, Schroeder A, Joanes M, Azeloglu EU, Sealfon R, Winfree S, Steck B, He Y, D'Agati VD, Iyengar R, Troyanskaya OG, Barisoni L, Gaut J, Zhang K, Laszik Z, Rovin B, Dagher PC, Sharma K, Sarwal M, Hodgin JB, Alpers CE, Kretzer M, Jain S, The Kidney Precision Medicine Project.
Physiol Genomics. 2020 Nov 16.

Cell shape regulates subcellular organelle location to control early Ca2+ signal dynamics in vascular smooth muscle cells.

Calizo RC, Bell MK, Ron A, Hu M, Bhattacharya S, Wong NJ, Janssen WGM, Perumal G, Pederson P, Scarlata S, Hone J, Azeloglu EU, Rangamani P, Iyengar R.
Sci Rep. 2020 Oct 20;10(1):17866.

Plasminogenuria is associated with podocyte injury, edema, and kidney dysfunction in incident glomerular disease.

Egerman MA, Wong JS, Runxia T, Mosoyan G, Chauhan K, Reyes-Bahamonde J, Anandakrishnan N, Wong NJ, Bagiella E, Salem F, Meliambro K, Li H, Azeloglu EU, Coca SG, Campbell KN, Raij L.
FASEB J. 2020 Dec;34(12):16191-16204.

Transcriptomic profiling of human cardiac cells predicts protein kinase inhibitor-associated cardiotoxicity.

van Hasselt JGC, Rahman R, Hansen J, Stern A, Shim JV, Xiong Y, Pickard A, Jayaraman G, Hu B, Mahajan M, Gallo JM, Goldfarb J, Sobie EA, Birtwistle MR, Schlessinger A, Azeloglu EU, Iyengar R.
Nat Commun . 2020 Sep 23;11(1):4809.

LIM-Nebulette Reinforces Podocyte Structural Integrity by Linking Actin and Vimentin Filaments.

Ge X, Zhang T. Yu, Muwonge AN, Anandakrishnan N, Wong NJ, Haydak JC, Reid JM, Fu J, Wong JS, Bhattacharya S, Cuttitta CM, Zhong F, Gordon RE, Salem F, Janssen W, Hone JC, Zhang A, Li H ,He JC, Gusella GL, Campbell KN, Azeloglu EU.
J Am Soc Nephrol . 2020 Oct;31(10):2372-2391.

The diabetic microenvironment causes mitochondrial oxidative stress in glomerular endothelial cells and pathological crosstalk with podocytes.

Casalena GA, Yu L, Gil R, Rodriguez S, Sosa S, Janssen W, Azeloglu EU, Leventhal JS, Daehn IS.
Cell Commun Signal . 2020 Jul 8;18(1):105.

Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes.

Zhong Y, Lee K, Deng Y, Ma Y, Chen Y, Li X, Wei C, Yang S, Wang T, Wong NJ, Muwonge AN, Azeloglu EU, Zhang W, Das B, He JC, Liu R.
Nat Commun. 2019 Oct 4;10(1):4523.

Wilm's tumor 1 promotes memory flexibility.

Mariottini C, Munari L, Gunzel E, Seco JM, Tzavaras N, Hansen J, Stern SA, Gao V, Aleyasin H, Sharmas A, Azeloglu EU, Hodes GE, Russo SJ, Huff V, Birtwistle MR, Blitzer RD, Alberini CM, Iyengar R.
Nat Commun. 2019 Aug 21;10(1):3756.

Endothelin receptor-A mediates degradation of the glomerular endothelial surface layer via pathologic crosstalk between activated podocytes and glomerular endothelial cells.

Eberfors K, Weiner RJ, Yu L, Azeloglu EU, Yi Z, Jia F, Zhang W, Baron MH, He JC, Haraldsson B, Daehn I.
Kidney Int. 2019 Oct;96(4):957-970.

Systems pharmacology-based integration of human and mouse data for drug repurposing to treat thoracic aneurysms.

Hansen J, Galatioto J, Caescu CI, Arnaud P, Calizo RC, Spronck B, Murtada SI, Borkar R, Weinberg A, Azeloglu EU, Bintanel-Morcillo M, Gallo JM, Humphrey JD, Jondeau G, Boileau C, Ramirez F, Iyengar R.
JCI Insight. 2019 Jun 6;4(11). pii: 127652.

Disruption of podocyte cytoskeletal biomechanics by dasatinib leads to nephrotoxicity.

Calizo RC, Bhattacharya S, van Hasselt JGC, Wei C, Wong JS, Wiener RJ, Ge X, Wong NJ, Lee JJ, Cuttitta CM, Jayaraman G, Au VH, Janssen W, Liu T, Li H, Salem F, Jaimes EA, Murphy B, Campbell KN, Azeloglu EU.
Nat Commun. 2019 May 3;10(1):2061.

Validating antibodies for quantitative western blot measurements with microwestern array.

Koch RJ, Barrette AM, Stern AD, Hu B, Bouhaddou M, Azeloglu EU, Iyengar R, Birtwistle MR.
Sci Rep. 2018 Jul 27;8(1):11329.

Dehomogenized elastic properties of heterogeneous layered materials in AFM indentation experiments.

Lee JJ, Rao S, Kaushik G, Azeloglu EU, Costa KD.
Biophys J. 2018 Jun 5;114(11):2717-2731.

Protein S protects against podocyte injury in diabetic nephropathy.

Zhong F*, Chen H*, Xie Y, Azeloglu EU, Wei C, Zhang W, Li Z, Chuang PY, Jim B, Li H, Elmastour F, Riyad JM, Weber T, Chen H, Wang Y, Zhang A, Jia W,
Lee K, He JC
J Am Soc Nephrol. 2018 May;29(5):1397-1410.

Cell shape information is transduced through tension-independent mechanisms.

Ron A*, Azeloglu EU*, Calizo RC, Hu M, Bhattacharya S, Chen Y, Jayaraman G, Lee S, Neves-Zaph SR, Li H, Gordon RE, He JC, Hone JC, Iyengar R.
Nat Commun. 2017 Dec 15;8(1):2145.

A comparison of mRNA sequencing with random primed and 3’-directed libraries.

Xiong Y, Hansen J, Shim J, Hu B, Jayaraman G, Sobie EA, Mahajan M, Iyengar R, Azeloglu EU* Birtwistle MR*
Sci Rep. 2017 Nov 7;7(1):14626.

Fragility of foot process morphology in kidney podocytes arises from chaotic spatial propagation of cytoskeletal instability.

Falkenberg CV, Azeloglu EU, Stothers M, Deerinck TJ, Chen Y, He JC, Ellisman MH, Hone JC, Iyengar R, Loew LM.
PLoS Comput Biol. 2017 Mar 16;13(3):e1005433.

A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction.

Hu M, Azeloglu EU, Ron A, Tran-Ba KH, Calizo RC, Tavassoly I, Bhattacharya S, Jayaraman G, Chen Y, Rabinovich V, Iyengar R, Hone JC, He JC, Kaufman
Sci Rep. 2017 Mar 6;7:43934.

Novel mutations in the inverted formin 2 gene of Chinese families contribute to focal segmental glomerulosclerosis.

Xie J*, Hao X*, Azeloglu EU, Ren H, Wang Z, Ma J, Liu J, Ma X, Wang W, Pan X, Zhang W, Zhong F, Li Y, Meng G, Kiryluk K, He JC, Gharavi AG, Chen N.
Kidney Int. 2015 Sep;88(3):593-604.

Interconnected regulatory motifs control podocyte morphology and kidney function.

Azeloglu EU*, Hardy S*, Eungdamrong NJ, Chen Y, Jayaraman G, Chuang PY, Fang W, Xiong H, Neves SR, Jain MR, Li H, Ma’ayan A, Gordon RE, He JC, Iyengar R.
Sci Signal. 2014 Feb 4;7(311):ra12.

Small molecule-mediated directed differentiation of human embryonic stem cells toward ventricular cardiomyocytes.

Karakikes I*, Senyei GD*, Hansen J, Kong C, Azeloglu EU, Francesca S, Lieu DK, Wang J, Ren L, Hulot JS, Iyengar R, Li RA, Hajjar RJ.
Stem Cells Trans Med. 2014 Jan;3(1):18-31.

Systems pharmacology of adverse event mitigation by drug combinations.

Zhao S, Nishimura T, Chen Y, Azeloglu EU, Gottesman O, Giannarelli C, Zafar MU, Benard L, Badimon JJ, Hajjar RJ, Goldfarb J, Iyengar R.
Sci Transl Med. 2013 Oct 9;5(206):206ra140.

Decoding information in cell shape.

Rangamani P, Lipshtat A, Azeloglu EU, Calizo RC, Hu M, Ghassemi S, Hone J, Scarlata S, Neves SR, Iyengar R.
Cell. 2013 Sep 12;154(6):1356-69.

The guidance of stem cell differentiation by substrate alignment and mechanical stimulation.

Subramony SD, Dargis BR, Castillo M, Azeloglu EU, Tracey MS, Su A, Lu HH.
Biomaterials. 2013 Mar;34(8):1942-53.

Cross-bridge cycling gives rise to spatiotemporal heterogeneity of dynamic subcellular mechanics in cardiac myocytes probed with atomic force microscopy.

Azeloglu EU, Costa KD.
Am J Physiol Heart Circ Physiol. 2010 Mar;298(3):H853-60.

Continuum modeling of biological tissue growth by cell division, and alteration of intracellular osmolytes and extracellular fixed charge density.

Ateshian GA, Costa KD, Azeloglu EU, Morrison B 3rd, Hung CT.
J Biomech Eng. 2009 Oct;131(10):101001.

Microscale control of stiffness in a cell-adhesive substrate using microfluidics-based lithography.

Cheung YK, Azeloglu EU, Shiovitz DA, Costa KD, Seliktar D, Sia SK.
Angew Chem Int Ed Engl. 2009;48(39):7188-92.

Increased myocyte content and mechanical function within a tissue-engineered myocardial patch following implantation.

Kelly DJ, Rosen AB, Schuldt AJ, Kochupura PV, Doronin SV, Potapova IA, Azeloglu EU, Badylak SF, Brink PR, Cohen IS, Gaudette GR.
Tissue Eng Part A. 2009 Aug;15(8):2189-201.

Atomic force microscope elastography reveals phenotypic differences in alveolar cell stiffness.

Azeloglu EU, Bhattacharya J, Costa KD.
J Appl Physiol (1985). 2008 Aug;105(2):652-61.

Engineered cardiac organoid chambers: toward a functional biological model ventricle.

Lee EJ, Kim DE, Azeloglu EU, Costa KD.
Tissue Eng Part A. 2008 Feb;14(2):215-25.

Heterogeneous transmural proteoglycan distribution provides a mechanism for regulating residual stresses in the aorta.

Azeloglu EU, Albro MB, Thimmappa VA, Ateshian GA, Costa KD.
Am J Physiol Heart Circ Physiol. 2008 Mar;294(3):H1197-205.

Mechanical heterogeneity of the rat hippocampus measured by atomic force microscope indentation.

Elkin BS, Azeloglu EU, Costa KD, Morrison B.
J Neurotrauma. 2007 May;24(5):812-22.

Accuracy and reproducibility of a subpixel extended phase correlation method to determine micron level displacements in the heart.

Kelly DJ, Azeloglu EU, Kochupura PV, Sharma GS, Gaudette GR.
Med Eng Phys. 2007 Jan;29(1):154-62

High resolution mechanical function in the intact porcine heart: mechanical effects of pacemaker location.

Azeloglu EU, Yun YH, Saltman AE, Krukenkamp IB, Chiang FP, Chen W, Gaudette GR.
J Biomech. 2006;39(4):717-25.

Tissue-engineered myocardial patch derived from extracellular matrix provides regional mechanical function.

Kochupura PV, Azeloglu EU, Kelly DJ, Doronin SV, Badylak SF, Krukenkamp IB, Cohen IS, Gaudette GR.
Circulation. 2005 Aug 30;112(9 Suppl):I144-9.

Delivery of a vector encoding mouse hyaluronan synthase 2 via a crosslinked hyaluronan film.

Kim AP, Yellen P, Yun YH, Azeloglu E, Chen W.
Biomaterials. 2005 May;26(13):1585-93.

Effects of ischemia on epicardial deformation in the passive rabbit heart.

Gaudette GR, Krukenkamp IB, Azeloglu EU, Saltman AE, Lense M, Todaro J, Chiang FP.
J Biomech Eng. 2004 Feb;126(1):70-5.

Determination of Regional Area Stroke Work with High Spatial Resolution in the Heart.

Gaudette GR, Azeloglu EU, Todaro BS, Saltman AE, Krukenkamp IB, Chiang FP.
Cardiovasc Eng. December 2002, Volume 2, Issue 4, pp 129–137.

Reviews and Book Chapters

Kidney tissue engineering for precision medicine.

Anandakrishnan N, Azeloglu EU.
Nat Rev Nephrol. 2020 Nov;16(11):623-624

The Library of Integrated Network-Based Cellular Signatures NIH Program: System-Level Cataloging of Human Cells Response to Perturbations.

Keenan AB, Jenkins SL, Jagodnik KM, Koplev S, He E, Torre D, Wang Z, Dohlman AB, Silverstein MC, Lachmann A, Kuleshov MV, Ma'ayan A, Stathias V, Terryn R, Cooper D, Forlin M, Koleti A, Vidovic D, Chung C, Schürer SC, Vasiliauskas J, Pilarczyk M, Shamsaei B, Fazel M, Ren Y, Niu W, Clark NA, White S, Mahi N, Zhang L, Kouril M, Reichard JF, Sivaganesan S, Medvedovic M, Meller J, Koch RJ, Birtwistle MR, Iyengar R, Sobie EA, Azeloglu EU, Kaye J, Osterloh J, Haston K, Kalra J, Finkbiener S, Li J, Milani P, Adam M, Escalante-Chong R, Sachs K, Lenail A, Ramamoorthy D, Fraenkel E, Daigle G, Hussain U, Coye A, Rothstein J, Sareen D, Ornelas L, Banuelos M, Mandefro B, Ho R, Svendsen CN, Lim RG, Stocksdale J, Casale MS, Thompson TG, Wu J, Thompson LM, Dardov V, Venkatraman V, Matlock A, Van Eyk JE, Jaffe JD, Papanastasiou M, Subramanian A, Golub TR, Erickson SD, Fallahi-Sichani M, Hafner M, Gray NS, Lin JR, Mills CE, Muhlich JL, Niepel M, Shamu CE, Williams EH, Wrobel D, Sorger PK, Heiser LM, Gray JW, Korkola JE, Mills GB, LaBarge M, Feiler HS, Dane MA, Bucher E, Nederlof M, Sudar D, Gross S, Kilburn DF, Smith R, Devlin K, Margolis R, Derr L, Lee A, Pillai A.
Cell Syst. 2018 Jan 24;6(1):13-24.